TY - JOUR AU - Oyong Imelda Ada, AU - Nkechi Egbe, AU - Gabriel Brian onwumere, AU - Yusuf Rabe, AU - Ugochukwu Ozojiorfor, AU - Abba Umar Hassan, AU - Yusuf Muhammad Sanyinna, PY - 2022/10/31 Y2 - 2024/03/28 TI - Mutations in the Quinolones Resistance Determining Regions of gyrA in Nosocomial Staphylococcus aureus and Salmonella typhi: Mut. in the Quinol. Resist. Determ. Reg. of gyrA in Nosocom. S. aureus & S. typh JF - Journal of Current Biomedical Research JA - jcbr VL - 2 IS - 5, September-October SE - Articles DO - 10.54117/jcbr.v2i5.2 UR - https://journals.unizik.edu.ng/jcbr/article/view/1214 SP - 415-422 AB - <div><p class="Default"><span lang="EN-US">In Nigeria, <em>Staphylococcus aureus </em>and <em>Salmonella typhi </em>are common causes of human infections and are also recognized as pathogens of public health significance. This study therefore, sought to determine the incidence and extent of fluoroquinolones resistance of <em>S. typhi </em>and <em>S. aureus </em>isolated from patients in Nigerian Defence Academy Hospital. A total of 60 samples obtained from patients with request for stool microscopy, culture and sensitivity, wound swabs, indoor air of surgical wards and swabbing of working benches were analyzed for the presence of <em>S. aureus </em>and <em>S. typhi</em>. The bacterial isolates were then subjected to antibiotic sensitivity testing using a modified Kirby-Bauer disk diffusion method. The antibiotic susceptibility patterns of <em>S. typhi </em>revealed that some of the isolates were resistant to two or more fluoroquinolones namely: ciprofloxacin, sparfloxacin, ofloxacin and pefloxacin. <em>S. aureus</em> on the other hand, also showed resistance to fluoroquinolones. The isolates that showed resistance to more fluoroquinolones were taken for molecular analysis. The genomic DNA was extracted and amplified using specific primer for <em>gyrA</em> by PCR, visualized using agarose gel electrophoresis and then sequenced. The amplicon sizes were 251bp respectively for each of the isolates. The detection of resistant pattern </span><span lang="EN-US">responsible for fluoroquinolones resistance showed that mutation had occurred. Mutation in nucleotide sequence was detected in <em>gyrA</em> gene of the fluoroquinolone resistant strains.</span></p></div> ER -