TY - JOUR AU - Ikpefan, Emmanuel AU - Omekudo, Oviemuno AU - Enwa, Felix PY - 2022/12/31 Y2 - 2024/03/29 TI - Antimicrobial and antioxidant studies of the methanol leaves extract of Cnestis ferruginea Dc (connaraceae) JF - Journal of Current Biomedical Research JA - jcbr VL - 2 IS - 6, November-December SE - Articles DO - 10.54117/jcbr.v2i6.9 UR - https://journals.unizik.edu.ng/jcbr/article/view/1307 SP - 683-699 AB - <p>The leaves of  <em>Cnestis ferruginea</em>  is used in the treatment of various ailment and diseases. This work is aimed at investigating the antimicrobial and antioxidant studies of the extract of <em>Cnestis ferruginea</em> DC (Connaraceae) leaves. The methanol extract was evaluated for the presence of phytochemicals. The methanol extract was evaluated for antimicrobial activities against <em>Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Candida albicans, </em>and <em>Aspergillus spp</em>. Thereafter, antioxidant activities were also carried out with DPPH (2,2- Diphenyl-1-picryl hydrazyl) and FRAP (Ferric reducing antioxidant power) methods using ascorbic acid as standard for DPPH and FRAP. The total phenolic content was determined using garlic acid and total flavonoids content was determined using quercetin as their standard respectively. The results indicate the presence of phytochemicals such as, tannins, alkaloids, terpenoids, saponins, cardiac glycosides, anthraquinones, and flavonoids. The results also indicate that all the organisms were sensitive to the various extract concentrations used (100 – 6.25 mg/mL). The minimum inhibitory concentration demonstrated by the organisms include, <em>E. coli</em> (12.5mg/mL), <em>B. subtilis </em>(6.25mg/mL)<em>, S. aureus </em>(12.5mg/mL),<em> P. aeruginosa </em>(6.25mg/mL), <em>C. Albicans </em>(100mg/mL),<em> and A. spp </em>(100mg/mL). The mean % inhibition for DPPH increases with increasing concentrations compared to FRAP that decreases with increasing concentrations. The total phenolic and flavonoids content of the crude extract of <em>C. ferruginea</em> leaves are 82.89 <u>+</u> 0.06 mg/GAE/g and 57.09 <u>+</u> 0.04 mg/QAE/g respectively. This study shows that the plant extract exhibited good antimicrobial and antioxidant activities against the test microorganisms.</p> ER -