Surface Thermodynamics Study and Spectrophotometric Data Analysis of Human Immunodeficiency Virus (HIV)-Antiviral Herbal Drug Coated Blood Interactions

Abstract

The surface thermodynamics study and spectrophotometric data analysis of human immunodeficiency virus (HIV)-antiviral herbal drug-coated blood interactions was carried out. Data of thermodynamic parameters were collected to measure their efficacy in line with the absorbance characteristics of HIV infected and uninfected blood. The experimental method involved the serial dilution of the five different antiviral drugs (two single Herbal, three-in-one combined herbal, one Highly Active Antiretroviral Therapy/Fixed Dose Combination and one single antiretroviral drugs) and their subsequent incubation with the blood samples collected from ten HIV infected persons who had already commenced treatment with one or more antiretroviral drugs, and ten HIV negative persons. Absorbance measurements using a digital Ultraviolet Visible MetaSpecAE1405031Pro Spectrophotometer at 25 set wavelengths were made and the peak absorbance data for various interacting systems were measured. The average peak absorbance values of different antiviral drugs interacted with HIV+ blood samples ranged from 1.82nm to 2.81nm at a constant 400Å. These fell within the visible range of the UV radiation which is 300-600 Angstrom. Average peak absorbance values of different antiviral drugs interacted with different blood components of HIV- blood samples ranged from 0.2nm to 2.05nm at a constant average of 350Å. The combined Hamaker coefficient (A132abs.) for HIV+ of -0.0031 with drug 1 shows effectiveness in repelling a HIV particle with a coating efficacy shown in corresponding positive sign of A131abs.of 0.1349 for HIV- samples. It is hoped that the use of the findings of this work in herbal drug design would yield good results in the fight against HIV and other viral diseases.