FTIR Analysis and Antimicrobial Evaluation OF Aqueous Ethanol Leaf Extract and Fractions oF Pavonia Snegalensis (Cav.) Liestner (Malvaceae)

Abstract

The leaves of Pavonia senegalensis have been used in African traditional medicine in treatment of ailments like bone and wound infections. In this study the FTIR analysis and antimicrobial evaluation of the extracts and fractions were carried out so as to provide a chemical fingerprint for the standardization of the plant and the scientific basis for the use of the plant in treatment of wounds and bone infections. FTIR analysis was conducted by scanning the samples in the mid-IR region (650-4000 cm^-1). The in-vitro antimicrobial activity of the leaf extract and fractions of plant was evaluated using S. aureus, E. coli, P. aeruginosa, S. Typi, S. pyrogens and Vancomycin reisitant enterooccci (VRE) as test organisms. The FTIR spectra of the plant extract and fractions revealed the presence various functional groups which include alkanes, alkenes, alkynes, aldehydes, ketones and alcohols/phenols. In the antimicrobial studies, the extract and fractions of the plant inhibited the growth of all the test organisms used in the study and were found to be bacteriostatic. In conclusion, the result of the study provide a biochemical fingerprint for the standardization of the plant and also justifies the ethnobotanical use of the plant in treatment of bone and wound infections.