Methanol root extract of Pausinystalia yohimbe, an aphrodisiac influences Morphology of sperm cells in male Wistar Rats

Authors

  • Adekunle Ojatula Olusegun Agagu University of Science and Technology, Okitipupa, Ondo State, Nigeria

DOI:

https://doi.org/10.54117/jcbr.v2i3.22

Keywords:

Keywords: Fertility enhancer, Medicinal plant, Normalities, Sperm-shape, Testicular integrity

Abstract

Abstract

T

 

The production and classification of sperm shape abnormalities in laboratory animals through administration of chemical agents, be it natural or synthetic, has in recent years developed into a very reliable, species and drug specific method of assay for testing the mutagenicity of phytotherapeutic agent. In this research, observed numbers, percentage and types of dysmorphisms induced by the oral administration of Pausinystalia yohimbe methanol root extract in varying doses in male wistar rats were investigated. Forty five rats were randomly divided into five groups of nine rats each. Rats in group I (control) were administered 1 mL/kg distilled water, group II received 5 mg/kg body weight sildenafil citrate, while those in groups III, IV, and V were given 25, 50, and 100 mg/kg body weight of the extract respectively for 14 days after which they were sacrificed and sperm cells were analysed for possible morphological abnormalities. In this study, oral administration of the extract has significant (p < 0.05) effect on the morphology of sperm cells, with sperm normalities decreases, while abnormalities increases when compared with the control groups. However, the abnormalities decreased dose dependently with slightly increase in normalities. The abnormal morphology ranged from double heads, tailless sperm, headless sperm, double tails, short sperm and bent tail in both extract and sildenafil citrate administered animals. The tailless sperm morphological changes had the highest rate of occurrence. The observation confirmed that mutagenicity of chemical agents, be it natural or synthetic, could be tested and compared using the sperm head-shape abnormality assay method, thereby predicting simple to complex dysmorphisms.

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Published

2022-06-30

How to Cite

Ojatula, A. (2022). Methanol root extract of Pausinystalia yohimbe, an aphrodisiac influences Morphology of sperm cells in male Wistar Rats. Journal of Current Biomedical Research, 2(3, May-June), 160–170. https://doi.org/10.54117/jcbr.v2i3.22