A Novel Approach on the Sensitivity of Preserved Plasmodium falciparum Positive RDT kits for the Molecular Detection of Pfmdr 1 and Pfcrt Genes in Pregnant Women

Abstract

Storing blood samples for molecular screening have become increasingly difficult due to unstable electricity and high cost of generators fuels. Samples often deteriorate giving false negative upon molecular analysis. This research was aimed at determining the sensitivity of preserved Plasmodium falciparum positive RDT kits for the molecular detection of Pfmdr 1 and Pfcrt genes among pregnant women in From February 2020 to May 2020, Sokoto, Nigeria. 200 pregnant patients who visited the hospital for ANC had their blood samples taken, and a questionnaire was given out. To detect Plasmodium falciparum, RDT kits tailored to its monoclonal antibody were utilized. P. falciparum positive Rapid Diagnostic Test Kits were air tightened with nylon and silica-preserved for three months in a closed cabinet. The preserved positive samples were utilized to extract DNA, and the Pfmdr1 and Pfcrt genes' existence was then checked using a PCR procedure. 4 samples tested positive for both genes, suggesting a 21.05 % prevalence, according to the results of the inquiry into the Pfcrt and Pfmdr1 genes. This study has revealed the sensitivity of preserved RDT kits (with silica) for DNA extraction. Researchers and molecular laboratories should be preserving positive RDT kits meant for DNA extraction and other molecular analysis as it is much easier to preserve and there is no cost involved.