Investigation Of Pro-fertility Actions Of “Female Corrective” Poly-herbal Medicine Formulation Using Female Wister Rat Model

Abstract

Plants contain numerous phytochemical compounds that may be used to cure or prevent diseases. In this study, the pro-fertility actions of “Female corrective” poly-herbal formulation were investigated using female Wister rat model. Phytochemicals, acute toxicities, body weight changes, and antioxidant and female reproductive hormonal studies were carried out. Serum levels of glutathione, superoxide dismutase and catalase were also evaluated. Micro well experiment was used to study the impact of the poly-herbal formulation on reproductive hormones. The phytochemical compounds present in the “Female corrective” were glycosides, saponins, tannins, flavonoids, steroids and terpenoids. In the median lethal dose estimation, no mortality nor obvious signs of toxicity were recorded at the dose of 88 mg/kg body weight. At a concentration of 4.4 mg/mL the body weights of the rats were increased in a dose dependent manner; 2.06, 4.11 and 8.22 mL/kg body weight gave changes in body weights of 17.13, 20.00 and 22.44% respectively after 14 days. Antioxidant effects were recorded as increase in serum concentration of glutathione, superoxide dismutase and catalase. Also, the serum levels of follicle stimulating hormone and luteinizing hormone were increased significantly (p ˂ 0.05) and dose dependently; at the same administered doses, serum levels of follicle stimulating hormone were 3.06 ± 0.06, 4.61 ± 0.05 and 5.25 ± 0.09 mIU/mL respectively and that of luteinizing hormone were 1.02 ± 0.10, 1.71 ± 0.23 and 2.08 ± 0.20 mIU/mL respectively. The possible mechanisms of the herbal drug formulation might include antioxidant as well as pro-gonadotropin activities