Preliminary study on isolation, purification and partial characterization of phospholipase A2 from a Nigerian Naja nigricolis venom

Authors

  • Yahaya Tijani
  • Muhammad Ibrahim Khala
  • Miriam Watafua
  • Afiniki Bitrus Mbya
  • Abubakar Shettima
  • Mohammed Abba Dige
  • Andrew Onu
  • Hassan Mohammed Khala
  • Bulama Burah
  • Tan Choo Hock
  • Hassan Zanna

DOI:

https://doi.org/10.54117/jcbr.v4i6.4

Keywords:

Naja nigricolis, Nigerian, isolation, Purification, Phospholipase A2

Abstract

Phospholipase A2 in Naja species of snake is an enzyme involved in venom toxicity. It hydrolyzes phospholipids, affecting cell membranes. Understanding its properties can contribute to venom research, offering insights into potential medical applications or antivenom development. In this study, isolation and purification of phospholipase A2 from Nigerian Naja nigricolis venom was achieved with the use of SDS-PAGE, Resource-S-Cation-Exchange Chromatography and Reverse-phase HPLC. The crude venom was also assessed for PLA2 optimum pH and temperature. A successful isolation and purification was achieved with a notable yield of 768 µg. The enzyme has a molecular weight of 13 kDa and exhibits optimal activity at a pH of 8.0 and a temperature of 500C. This purification method proves effective in isolating the enzyme, offering potential applications in research and furthering our understanding of the Nigerian Naja nigricolis venom phospholipase A2. These findings provide valuable insights into the characteristics of Naja nigricolis phospholipase A2 for potential applications in venomics and antivenomics.

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Published

2024-12-30

Issue

Vol. 4 No. 6, November-December (2024): Journal of Current Biomedical Research

Section

Articles

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License

Copyright (c) 2024 Yahaya Tijani, Muhammad Ibrahim Khala , Miriam Watafua, Afiniki Bitrus Mbya, Abubakar Shettima, Mohammed Abba Dige, Andrew Onu, Hassan Mohammed Khala , Bulama Burah, Tan Choo Hock, Hassan Zanna

All articles in JCBR are published under CC BY 4.0. Authors retain copyright of their articles. The Journal of Current Biomedical Research (JCBR) publishes all articles under the Creative Commons Attribution 4.0 International license (CC BY 4.0). This license permits use, sharing, adaptation, distribution, and reproduction in any medium or format, for any purpose, provided appropriate credit is given to the original author(s) and the source, a link to the license is provided, and any changes are indicated. The Version of Record should be cited with its DOI.
License: https://creativecommons.org/licenses/by/4.0/ 

How to Cite

Preliminary study on isolation, purification and partial characterization of phospholipase A2 from a Nigerian Naja nigricolis venom. (2024). Journal of Current Biomedical Research, 4(6, November-December), 1906-1917. https://doi.org/10.54117/jcbr.v4i6.4