Preliminary study on isolation, purification and partial characterization of phospholipase A2 from a Nigerian Naja nigricolis venom

Abstract

Phospholipase A₂ in Naja species of snake is an enzyme involved in venom toxicity. It hydrolyzes phospholipids, affecting cell membranes. Understanding its properties can contribute to venom research, offering insights into potential medical applications or antivenom development. In this study, isolation and purification of phospholipase A₂ from Nigerian Naja nigricolis venom was achieved with the use of SDS-PAGE, Resource-S-Cation-Exchange Chromatography and Reverse-phase HPLC. The crude venom was also assessed for PLA₂ optimum pH and temperature. A successful isolation and purification was achieved with a notable yield of 768 µg. The enzyme has a molecular weight of 13 kDa and exhibits optimal activity at a pH of 8.0 and a temperature of 50⁰C. This purification method proves effective in isolating the enzyme, offering potential applications in research and furthering our understanding of the Nigerian Naja nigricolis venom phospholipase A₂. These findings provide valuable insights into the characteristics of Naja nigricolis phospholipase A₂ for potential applications in venomics and antivenomics.