THE USE OF GENE XPERT MTB/MTB RIF ASSAY IN THE DIAGNOSIS OF EXTRA-PULMONARY TUBERCULOSIS AT NAUTH, NNEWI

Authors

  • ANYABOLU Arthur Ebelenna Department of Internal Medicine, College of Health Sciences, Faculty of Medicine, Nnamdi Azikiwe University, Anambra State, Nigeria.
  • USHIE Simon Nkpeh TB (DOTS) Laboratory Unit, Department of Medical Microbiology, Nnamdi Azikiwe University Teaching Hospital, Nnewi, Anambra State, Nigeria. https://orcid.org/0000-0002-7429-1723
  • OKONKWO Robert Chuks TB (DOTS) Laboratory Unit, Department of Medical Microbiology, Nnamdi Azikiwe University Teaching Hospital, Nnewi, Anambra State, Nigeria.
  • ENEMUO Emeka Hyacinth Department of Internal Medicine, College of Health Sciences, Faculty of Medicine, Nnamdi Azikiwe University, Anambra State, Nigeria.
  • UFOAROH Chinyelu Uchenna Department of Internal Medicine, College of Health Sciences, Faculty of Medicine, Nnamdi Azikiwe University, Anambra State, Nigeria.
  • CHUKUANUKWU Rebecca Chinyelu Department of Medical Laboratory Science, Faculty of Health Sciences and Technology, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria.
  • CHUKWUMA George Okechukwu Department of Medical Laboratory Science, Faculty of Health Sciences and Technology, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria.

Keywords:

Extra pulmonary tuberculosis, ZN microscopy, GeneXpertMTB/RIF, Aspirates

Abstract

Introduction: About 15–20% of active tuberculosis cases may spread outside the lungs to affect other parts of the body causing what is known as extra pulmonary tuberculosis (EPTB). Cases of Extra Pulmonary Tuberculosis are equally prevalent in all the Tuberculosis  high burden zones. Information on Extra pulmonary Tuberculosis is scarce in Nigeria despite being high burden TB country. Existing tests for diagnosis of extra pulmonary Tuberculosis are limited in accuracy and turn-around time and often require invasive procedures and special expertise.

Aim: This study aimed to assess the utility of the Gene Xpert MTB/RIF in the diagnosis of extra pulmonary Tuberculosis and document  the burden of Extra Pulmonary TB in NAUTH, Nnewi, Nigeria.

Materials and Methods: Extra pulmonary samples (comprising pleural aspirates, ascetic fluids, cerebrospinal fluids, gastric lavage, pus, pleural fluids, scrotal aspirates, hydrocele fluids and biopsy materials) taken from 288 patients accessing the Nnamdi Azikiwe University Teaching Hospital, Nnewi were examined using Ziehl Neelsen smear microscopy and Xpert MTB/RIF assay.

Results: Gene Xpert MTB/RIF assay detected MTB in 18 (6.3%) of 288 EPTB suspects against 8 (2.8%) of 288 detected by Ziehl Nelseen AFB microscopy.

Conclusion: The inclusion of Gene Xpert MTB/RIF assay method in routine diagnostic protocol for EPTB is a welcome development due to its faster turnaround time and higher sensitivity compared to smear microscopy.

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Published

31-03-2023

How to Cite

ANYABOLU, A. E., USHIE, S. N., OKONKWO, R. C., ENEMUO, E. H., UFOAROH, C. U., CHUKUANUKWU , R. C., & CHUKWUMA, G. O. (2023). THE USE OF GENE XPERT MTB/MTB RIF ASSAY IN THE DIAGNOSIS OF EXTRA-PULMONARY TUBERCULOSIS AT NAUTH, NNEWI. Journal of Biomedical Investigation, 11(1), 64–70. Retrieved from https://journals.unizik.edu.ng/jbi/article/view/1992

Issue

Vol. 11 No. 1 (2023)

Section

Articles

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