MOLECULAR DETECTION OF CEFOTAXIMASE MUNICH (CTX-M) RESISTANT ACINETOBATER BAUMANNII IN A TERTIARYCARE HOSPITAL IN SOUTHEAST NIGERIA

Authors

  • OKORO, Akachukwu Egwu Department of Medical Microbiology and Parasitology, Faculty of Health Sciences and Technology, Nnamdi Azikiwe University, Nnewi Campus, Anambra State, Nigeria.
  • AKUJOBI, Comfort Nne Department of Medical Microbiology and Parasitology, Faculty of Health Sciences and Technology, Nnamdi Azikiwe University, Nnewi Campus, Anambra State, Nigeria.
  • AGHANYA, Nnaemeka Iloduba Department of Medical Microbiology and Parasitology, Faculty of Health Sciences and Technology, Nnamdi Azikiwe University, Nnewi Campus, Anambra State, Nigeria.
  • UKIBE, Solomon N. Department of Medical Microbiology and Parasitology, Faculty of Health Sciences and Technology, Nnamdi Azikiwe University, Nnewi Campus, Anambra State, Nigeria.
  • NNEMELU, Perpetua Onyinye Department of Medical Microbiology and Parasitology, Faculty of Health Sciences and Technology, Nnamdi Azikiwe University, Nnewi Campus, Anambra State, Nigeria.
  • OKWESILEZE, Chineyenwa Vivian Department of Medical Microbiology and Parasitology, Faculty of Health Sciences and Technology, Nnamdi Azikiwe University, Nnewi Campus, Anambra State, Nigeria.
  • EZEAMA, Jesse Chibuike Department of Medical Microbiology and Parasitology, Faculty of Health Sciences and Technology, Nnamdi Azikiwe University, Nnewi Campus, Anambra State, Nigeria.
  • AKUAKOLAM, Ikenna Macmagnus Department of Medical Microbiology and Parasitology, Faculty of Health Sciences and Technology, Nnamdi Azikiwe University, Nnewi Campus, Anambra State, Nigeria.
  • AJUBA, Ijeoma Maryrose Department of Medical Microbiology and Parasitology, Faculty of Health Sciences and Technology, Nnamdi Azikiwe University, Nnewi Campus, Anambra State, Nigeria.

Keywords:

Multidrug resistance, Acinetobacter baumannii, hospital infections

Abstract

Background: Acinetobacter baumannii (A. baumannii) is one of the numerous organisms responsible for multidrug resistant, hospital acquired infections in many tertiary health care institutions worldwide.

Aim: To detect the presence of cefotaximase (CTX) resistant A. baumannii in NAUTH Nnewi, Southeast, Nigeria.

Materials and Methods: Clinical isolates of Gram-negative bacilli (n=210) were obtained from several clinical specimens. All isolates  were phenotypically identified in the laboratory using standard cultural methods. Antimicrobial susceptibility tests were performed on  all A. baumannii species detected using the Kirby Bauer disc diffusion technique. Isolates resistant to any of the 3rd generation Cephalosporins were screened for ESBL activity using the Double disc synergy test. Polymerase Chain Reaction wasthen used to detect  the presence of CTX gene from the ESBL positive isolates.

Results: Out of the 210 Gram-negative bacilli, 20 (9.5%) were Acinetobacter species. The organisms were most resistant to amoxicillin-clavulanic acid and the 3rd generation cephalosporins. Cefotaximase (CTX) gene was observed in 3/20 (5%) of the Acinetobacter species.

Conclusion: Multidrug resistant Acinetobacter baumannii was prevalent in the study area, although the rate of CTX production was  relatively low. Consequently strict application of good infection prevention and control measures in the hospital setting is necessary to  curb the menace associated with this organism.

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Published

31-03-2023

How to Cite

OKORO, A. E., AKUJOBI, C. N., AGHANYA, N. I., UKIBE, S. N., NNEMELU, P. O., OKWESILEZE, C. V., EZEAMA, J. C., AKUAKOLAM, I. M., & AJUBA, I. M. (2023). MOLECULAR DETECTION OF CEFOTAXIMASE MUNICH (CTX-M) RESISTANT ACINETOBATER BAUMANNII IN A TERTIARYCARE HOSPITAL IN SOUTHEAST NIGERIA. Journal of Biomedical Investigation, 11(1), 41–46. Retrieved from https://journals.unizik.edu.ng/jbi/article/view/1989

Issue

Vol. 11 No. 1 (2023)

Section

Articles